Vasan Venugopalan, Sc.D.
University of California, Irvine
Laser-Generated Microtsunamis for Targeted Cell Lysis, Molecular Delivery and Mechanotransduction (more ...)
Laser-Generated Microtsunamis for Targeted Cell Lysis, Molecular Delivery and Mechanotransduction (hide ...)
Pulsed laser microbeam irradiation provides powerful tool for cell manipulation and modification due to its ability to deposit energy with high spatial localization. Often, such irradiation leads to plasma formation. Apart from free electron generation, plasma formation often results in local vaporization, shock wave emission, and cavitation bubble formation, expansion, and collapse. Our research has shown that when using nanosecond and picosecond laser pulses at visible and NIR wavelengths the stress waves produced by the cavitation bubble dynamics, which we term microtsunamis, are principally responsible for the resulting cellular effects. First, I will discuss the uses of laser-generated microtsunamis in standard applications of targeted cell lysis and optoporation. Second, I will provide results demonstrating the ability of laser-generated microtsunamis to stimulate cellular mechanotransduction and motivate potential applications for high throughput drug screening and basic biological studies. In both instances, I will illustrate the value of quantitative modeling as a means to connect the applied physical perturbations to cellular responses.
Bio: Vasan Venugopalan is Professor and Chair of the Department of Chemical Engineering & Materials Science at the University of California, Irvine. He also holds joint appointments in the Departments of Biomedical Engineering, Mechanical & Aerospace Engineering, and Beckman Laser Institute & Medical Clinic at UCI. His lab focuses on basic and applied research regarding the uses of pulsed laser microbeams in cell biology and biotechnology as well as the development of novel, open-source, computational tools to model light transport in cells and tissues. He has published over 60 peer-reviewed papers. He also serves Associate Editor of the OSA journals Optics Express (2009-present) and Biomedical Optics Express (2010-present).
Biophotonics Seminar, Light Hall, Room 202